New hardware and tools for macromolecular cryocrystallography
نویسندگان
چکیده
منابع مشابه
Macromolecular Cryocrystallography
The current techniques for X-ray-diffraction data collection from macromolecular crystals at cryogenic temperatures are reviewed. The development of the experimental methods is outlined and the basic concepts pertaining to radiation damage and cryoprotection are summarized. Emphasis is placed on the practical aspects important to the success of the techniques, and a detailed account of these is...
متن کاملPractical macromolecular cryocrystallography
Cryocrystallography is an indispensable technique that is routinely used for single-crystal X-ray diffraction data collection at temperatures near 100 K, where radiation damage is mitigated. Modern procedures and tools to cryoprotect and rapidly cool macromolecular crystals with a significant solvent fraction to below the glass-transition phase of water are reviewed. Reagents and methods to hel...
متن کاملMacromolecular Cryocrystallography: Cooling, Mounting, Storage and Transportation of Crystals
Simple methods are presented for handling, mounting, storage and transportation of crystals at cryogenic temperatures. They are easy to learn and have a number of technical and operational advantages over currently popular methods. In particular, the temperature of the crystal throughout all manipulations is known; it is shown never to rise above that of the warmest component of the cryogenic s...
متن کاملMacromolecular cryocrystallography--methods for cooling and mounting protein crystals at cryogenic temperatures.
Cryocrystallography is routinely used in macromolecular crystallography laboratories. The main advantage of X-ray diffraction data collection near 100K is that crystals display much less radiation damage than seen at room temperature. Techniques and tools are described to facilitate cryoprotecting and flash-cooling crystals for data collection.
متن کاملRadioprotectant screening for cryocrystallography.
Radiation damage continues to present a problem to crystallographers using cryocooled protein crystals at third-generation synchrotrons. Free-radical scavengers have been suggested as a possible means of reducing the rate of this damage. The screening of a large number of potential radioprotectants was undertaken with an online microspectrophotometer using cystine and cysteine to model protein ...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2002
ISSN: 0108-7673
DOI: 10.1107/s0108767302095430